Human Interleukin 16,IL-16 ELISA KIT
Product Details
| Target Name |
interleukin 16 (lymphocyte chemoattractant factor) |
| Alternative Names |
HGNC:5980 ELISA Kit; HsT19289 ELISA Kit; IL-16 ELISA Kit; IL16 ELISA Kit; IL16_HUMAN ELISA Kit; Interleukin 16 precursor ELISA Kit; Interleukin-16 ELISA Kit; LCF ELISA Kit; Lymphocyte chemoattractant factor ELISA Kit; Neuronal interleukin 16 ELISA Kit; NIL16 ELISA Kit; prIL 16 ELISA Kit; PrIL16 ELISA Kit; Prointerleukin 16 ELISA Kit |
| Abbreviation |
IL16 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
84 |
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| Range % |
81-88 |
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| 1:8 |
Average % |
89 |
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| Range % |
81-96 |
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| Recovery |
| The recovery of human IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
101 |
94-101 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
1.774 |
1.848 |
1.811 |
1.644 |
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| 5 |
1.003 |
1.014 |
1.009 |
0.842 |
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| 2.5 |
0.666 |
0.687 |
0.677 |
0.510 |
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| 1.25 |
0.401 |
0.412 |
0.407 |
0.240 |
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| 0.625 |
0.289 |
0.287 |
0.288 |
0.121 |
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| 0.312 |
0.245 |
0.253 |
0.249 |
0.082 |
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| 0.156 |
0.211 |
0.224 |
0.218 |
0.051 |
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| 0 |
0.163 |
0.170 |
0.167 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
