Name: Human Interleukin 18,IL-18 ELISA KIT
Brand: BioChain
Rating: 2 (28 reviews)

Product Details

Description	The Human Interleukin 18 (IL-18) ELISA Kit is designed to measure IL-18 biological samples, including serum, plasma, urine, tissue homogenates, in a quantitative fashion. This kit has been verified by multiple tests with advantages of high sensitivity, strong specificity, precision less than 10%, and lot-to-lot consistency. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction as well as colorimetric measurement, the levels of IL-18 in the sample can be calculated. IL-18 is a proinflammatory cytokine belonging to the IL-1 superfamily. It was initially described as an IFN-γ-inducing factor derived from anti-CD3-stimulated T-helper (Th) 1 cell. IL-18 plays a critical role in inflammation and the host defense against microbes. IL-18/IL-18R binding activates adaptor molecule MyD88, which subsequently stimulates the activation of TRAF6 and IRAK1-4, inducing the occurrence of the MAPK and NF-κB pathways that upregulate the expression of various inflammatory cytokines.
Target Name	interleukin 18 (interferon-gamma-inducing factor)
Alternative Names	Iboctadekin ELISA Kit; IFN gamma inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; IGIF ELISA Kit; IL 1 gamma ELISA Kit; IL 18 ELISA Kit; IL 1g ELISA Kit; IL-1 gamma ELISA Kit; IL-18 ELISA Kit; IL1 gamma ELISA Kit; IL18 ELISA Kit; IL18 protein ELISA Kit; IL18_HUMAN ELISA Kit; IL1F4 ELISA Kit; IL1g ELISA Kit; IL1gamma ELISA Kit; ILIF4 ELISA Kit; Interferon gamma inducing factor ELISA Kit; Interferon gamma-inducing factor ELISA Kit; Interleukin 1 gamma ELISA Kit; Interleukin 18 (interferon-gamma-inducing factor) ELISA Kit; Interleukin 18 ELISA Kit; Interleukin-1 gamma ELISA Kit; Interleukin-18 ELISA Kit; Interleukin18 ELISA Kit; MGC12320 ELISA Kit
Abbreviation	IL18
Species	Homo sapiens (Human)
Sample Types	serum, plasma, urine, tissue homogenates
Detection Range	31.25 pg/mL-2000 pg/mL
Sensitivity	7.8 pg/mL
Assay Time	1-5h
Sample Volume	50-100ul
Detection Wavelength	450 nm
Research Area	Immunology
Assay Principle	quantitative
Measurement	Sandwich
Materials provided	A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human IL-18 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-labeledIL-18 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody. HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution. HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution. Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration. Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances. TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual
Materials not provided	A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Squirt bottle, manifold dispenser, or automated microplate washer. Absorbent paper for blotting the microtiter plate. 100ml and 500ml graduated cylinders. Deionized or distilled water. Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips. Timer Test tubes for dilution.
Storage	Store at 2-8°C. Please refer to protocol.
Lead Time	3-5 working days

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Human Interleukin 18,IL-18 ELISA KIT

Product Details