Human Interleukin 21,IL-21 ELISA Kit
Product Details
| Target Name |
interleukin 21 |
| Alternative Names |
CVID11 ELISA Kit; IL 21 ELISA Kit; IL-21 ELISA Kit; Il21 ELISA Kit; IL21_HUMAN ELISA Kit; Interleukin 21 ELISA Kit; Interleukin-21 ELISA Kit; interleukin-21 isoform ELISA Kit; Interleukin21 ELISA Kit; OTTHUMP00000164088 ELISA Kit; Za11 ELISA Kit |
| Abbreviation |
IL21 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-99 |
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| 1:4 |
Average % |
87 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
94 |
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| Range % |
87-100 |
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| Recovery |
| The recovery of human IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
101 |
85-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.445 |
2.345 |
2.395 |
2.250 |
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| 100 |
1.864 |
1.764 |
1.814 |
1.669 |
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| 50 |
1.162 |
1.151 |
1.157 |
1.012 |
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| 25 |
0.723 |
0.713 |
0.718 |
0.573 |
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| 12.5 |
0.432 |
0.422 |
0.427 |
0.282 |
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| 6.25 |
0.315 |
0.305 |
0.310 |
0.165 |
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| 3.12 |
0.222 |
0.212 |
0.217 |
0.072 |
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| 0 |
0.145 |
0.144 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
