Human Interleukin 23,IL-23 ELISA Kit
Product Details
| Target Name |
Interleukin 23,IL-23 |
| Alternative Names |
N/A |
| Abbreviation |
IL-23 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-106 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
82-96 |
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| EDTA plasma (n=4) |
93 |
89-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.608 |
2.507 |
2.558 |
2.415 |
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| 200 |
1.865 |
1.764 |
1.815 |
1.672 |
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| 100 |
1.207 |
1.186 |
1.197 |
1.054 |
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| 50 |
0.801 |
0.790 |
0.796 |
0.653 |
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| 25 |
0.437 |
0.448 |
0.443 |
0.300 |
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| 12.5 |
0.301 |
0.312 |
0.307 |
0.164 |
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| 6.25 |
0.228 |
0.234 |
0.231 |
0.088 |
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| 0 |
0.142 |
0.143 |
0.143 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
