Human Interleukin 25,IL-25 ELISA kit
Product Details
| Target Name |
interleukin 25 |
| Alternative Names |
IL 17E ELISA Kit; IL 25 ELISA Kit; IL-17E ELISA Kit; IL-25 ELISA Kit; IL17e ELISA Kit; IL25 ELISA Kit; IL25_HUMAN ELISA Kit; Interleukin 17E ELISA Kit; Interleukin 25 ELISA Kit; Interleukin-17E ELISA Kit; Interleukin-25 ELISA Kit; Interleukin17E ELISA Kit; Interleukin25 ELISA Kit; OTTHUMP00000027946 ELISA Kit; OTTHUMP00000246238 ELISA Kit; UNQ3120/PRO10272 ELISA Kit |
| Abbreviation |
IL25 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, cell culturesupernates, urine, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
87-98 |
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| 1:2 |
Average % |
97 |
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| Range % |
90-103 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-106 |
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| 1:8 |
Average % |
98 |
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| Range % |
94-103 |
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| Recovery |
| The recovery of human IL-25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-89 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.892 |
2.752 |
2.822 |
2.711 |
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| 2000 |
2.299 |
2.179 |
2.239 |
2.128 |
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| 1000 |
1.645 |
1.599 |
1.622 |
1.511 |
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| 500 |
0.982 |
1.002 |
0.992 |
0.881 |
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| 250 |
0.561 |
0.602 |
0.582 |
0.471 |
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| 125 |
0.350 |
0.366 |
0.358 |
0.247 |
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| 62.5 |
0.268 |
0.275 |
0.272 |
0.161 |
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| 0 |
0.109 |
0.112 |
0.111 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
