Human Interleukin 28B(IL-28B) ELISA kit
Product Details
| Target Name |
Interleukin 28B(IL-28B) |
| Alternative Names |
Cytokine ZCYTO22 ELISA Kit; IFN-lambda-3 ELISA Kit; IFN-lambda-4 ELISA Kit; IL-28B ELISA Kit; IL-28C ELISA Kit; IL28B ELISA Kit; IL28B_HUMAN ELISA Kit; IL28C ELISA Kit; Interferon lambda-3 ELISA Kit; Interferon lambda-4 ELISA Kit; interleukin 28B (interferon; lambda 3) ELISA Kit; Interleukin 28C ELISA Kit; Interleukin-28B ELISA Kit; Interleukin-28C ELISA Kit |
| Abbreviation |
IL-28B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernats, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-92 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-105 |
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| 1:8 |
Average % |
96 |
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| Range % |
93-99 |
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| Recovery |
| The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
100 |
95-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.224 |
2.239 |
2.232 |
2.047 |
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| 50 |
1.509 |
1.534 |
1.522 |
1.337 |
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| 25 |
1.023 |
1.036 |
1.030 |
0.845 |
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| 12.5 |
0.688 |
0.699 |
0.694 |
0.509 |
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| 6.25 |
0.474 |
0.490 |
0.482 |
0.297 |
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| 3.12 |
0.387 |
0.374 |
0.381 |
0.196 |
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| 1.56 |
0.281 |
0.297 |
0.289 |
0.104 |
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| 0 |
0.184 |
0.186 |
0.185 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
