Human Interleukin 32(IL-32) ELISA Kit
Product Details
| Target Name |
interleukin 32 |
| Alternative Names |
IL 32alpha ELISA Kit; IL 32beta ELISA Kit; IL 32delta ELISA Kit; IL 32gamma ELISA Kit; IL-32 ELISA Kit; IL32 ELISA Kit; IL32_HUMAN ELISA Kit; Interleukin 32 ELISA Kit; Interleukin 32 small ELISA Kit; Interleukin 32 theta ELISA Kit; Interleukin-32 ELISA Kit; interleukin-32 eta ELISA Kit; Natural killer cell transcript 4 ELISA Kit; Natural killer cells protein 4 ELISA Kit; NK 4 ELISA Kit; NK4 ELISA Kit; OTTHUMP00000236040 ELISA Kit; OTTHUMP00000236041 ELISA Kit; OTTHUMP00000236042 ELISA Kit; OTTHUMP00000236043 ELISA Kit; OTTHUMP00000236044 ELISA Kit; OTTHUMP00000236045 ELISA Kit; OTTHUMP00000236046 ELISA Kit; OTTHUMP00000236047 ELISA Kit; OTTHUMP00000236048 ELISA Kit; OTTHUMP00000236049 ELISA Kit; OTTHUMP00000236050 ELISA Kit; OTTHUMP00000236051 ELISA Kit; OTTHUMP00000236052 ELISA Kit; OTTHUMP00000236053 ELISA Kit; OTTHUMP00000236054 ELISA Kit; OTTHUMP00000241545 ELISA Kit; OTTHUMP00000241602 ELISA Kit; OTTHUMP00000241603 ELISA Kit; OTTHUMP00000241604 ELISA Kit; OTTHUMP00000241605 ELISA Kit; OTTHUMP00000241606 ELISA Kit; OTTHUMP00000241607 ELISA Kit; OTTHUMP00000241608 ELISA Kit; OTTHUMP00000241609 ELISA Kit; OTTHUMP00000241610 ELISA Kit; OTTHUMP00000241611 ELISA Kit; OTTHUMP00000241612 ELISA Kit; TAIF ELISA Kit; TAIFa ELISA Kit; TAIFb ELISA Kit; TAIFc ELISA Kit; TAIFd ELISA Kit; Tumor necrosis factor alpha-inducing factor ELISA Kit |
| Abbreviation |
IL32 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
312.5 pg/mL-20000 pg/mL |
| Sensitivity |
78.125 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
89 |
|
| Range % |
82-97 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
94-104 |
|
| 1:4 |
Average % |
97 |
|
| Range % |
90-105 |
|
| 1:8 |
Average % |
87 |
|
| Range % |
82-92 |
|
|
| Recovery |
| The recovery of human GROα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-97 |
|
| EDTA plasma (n=4) |
101 |
96-106 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20000 |
2.801 |
2.752 |
2.777 |
2.627 |
|
| 10000 |
2.440 |
2.279 |
2.360 |
2.210 |
|
| 5000 |
1.784 |
1.789 |
1.787 |
1.637 |
|
| 2500 |
0.961 |
1.002 |
0.982 |
0.832 |
|
| 1250 |
0.547 |
0.575 |
0.561 |
0.411 |
|
| 625 |
0.338 |
0.346 |
0.342 |
0.192 |
|
| 312.5 |
0.234 |
0.225 |
0.230 |
0.080 |
|
| 0 |
0.146 |
0.154 |
0.150 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
