The Human Interleukin 35 (IL-35) ELISA Kit is specifically designed to standardize the detection of human IL-35 in multiple biological solutions, including serum, cell culture supernates, urine, and tissue homogenates. This kit employs the Sandwich-ELISA mechanism to quantitatively measure the concentration of IL-35. The IL-35 in the sample and standard is bound in a "sandwich" format by the capture antibody pre-coated on the microwell plate wells and subsequently added Biotin-labeled IL-35 antibody. The HRP-avidin is added and conjugated to the biotinylated IL-35 antibody, allowing for a colorimetric reaction to ensue upon TMB substrate addition. HRP-TMB interaction yields a blue color. The color development is terminated, and solution color changes from blue to yellow. The intensity is positively representative to the IL-35 concentration.
This kit has been professionally verified with reliable quality, high sensitivity, strong specificity, good linearity, recovery of 87-97%, precision less than 10%, and consistency between batches. See more details on the product instructions.
IL35 is cytokine generated by regulatory B cells (Bregs) and belongs to the IL12 family. It possesses immunosuppressive functions. IL-35 forms via heterodimerization of p35 and Ebi3 and signals in naïve CD4+ T cells through the IL-35R, activating STAT1 and STAT4 signaling pathways. IL-35 upregulation has been found in lymphoma cells and lung cancer and is associated with poor outcome in cases of leukemia, colorectal, and pancreatic cancer.
