Human Interleukin 37(IL-37) ELISA Kit
Product Details
| Target Name |
interleukin 1 family, member 7 (zeta) |
| Alternative Names |
FIL1 ELISA Kit; FIL1 zeta ELISA Kit; FIL1(ZETA) ELISA Kit; FIL1Z ELISA Kit; IL 1 zeta ELISA Kit; IL 1F7 ELISA Kit; IL 1F7b (IL 1H4; IL 1H; IL 1RP1) ELISA Kit; IL 1H4 ELISA Kit; IL 1RP1 ELISA Kit; IL 1X ELISA Kit; IL 1X protein ELISA Kit; IL-1 zeta ELISA Kit; IL-1F7 ELISA Kit; IL-1H ELISA Kit; IL-1H4 ELISA Kit; IL-1RP1 ELISA Kit; IL-1X ELISA Kit; IL-37 ELISA Kit; IL1F7 (canonical product IL 1F7b) ELISA Kit; IL1F7 ELISA Kit; IL1F7_HUMAN ELISA Kit; IL1H4 ELISA Kit; IL1RP1 ELISA Kit; Interleukin 1 family member 7 ELISA Kit; interleukin 1 family; member 7 (zeta) ELISA Kit; Interleukin 1 homolog 4 ELISA Kit; Interleukin 1 related protein ELISA Kit; Interleukin 1 superfamily z ELISA Kit; Interleukin 1 zeta ELISA Kit; Interleukin 37 ELISA Kit; Interleukin-1 family member 7 ELISA Kit; Interleukin-1 homolog 4 ELISA Kit; Interleukin-1 zeta ELISA Kit; Interleukin-1-related protein ELISA Kit; Interleukin-23 ELISA Kit |
| Abbreviation |
IL37 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
81-93 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
98 |
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| Range % |
88-103 |
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| Recovery |
| The recovery of human IL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
86-100 |
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| EDTA plasma (n=4) |
94 |
84-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
1.654 |
1.605 |
1.630 |
1.468 |
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| 1000 |
1.219 |
1.233 |
1.226 |
1.064 |
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| 500 |
0.854 |
0.892 |
0.873 |
0.711 |
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| 250 |
0.562 |
0.581 |
0.572 |
0.410 |
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| 125 |
0.359 |
0.379 |
0.369 |
0.207 |
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| 62.5 |
0.268 |
0.280 |
0.274 |
0.112 |
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| 31.25 |
0.218 |
0.213 |
0.216 |
0.054 |
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| 0 |
0.165 |
0.159 |
0.162 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
