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Human Interleukin 4,IL-4 ELISA KIT

Product Details

Description
The product CSB-E04633h is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of human interleukin 4 (IL-4) in biological samples, including serum, tissue homogenates, cell culture supernates, and saliva. Based on the Sandwich-ELISA technique in combination with enzyme-substrate chromogenic reaction, the IL-4 levels can be calculated according to the standard curve. This Human IL-4 ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 10%, good linearity, recovery of 90%-100%, and lot-to-lot consistency. IL-4 is a multifaceted anti-inflammatory cytokine and possesses anti-angiogenic capabilities. IL-4 suppresses tumor growth by inhibiting angiogenesis and also blocks corneal neovascularization induced by the basic fibroblast growth factor. IL-4 signaling via the IL-4R orchestrates type II immunity to helminth infections and toxins. Takashi Baba etc. revealed that the IL-4/IL-4R axis controlled tube formation .
Target Name interleukin 4
Alternative Names B cell growth factor 1 ELISA Kit; B cell IgG differentiation factor ELISA Kit; B Cell Stimulatory Factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BCGF 1 ELISA Kit; BCGF1 ELISA Kit; Binetrakin ELISA Kit; BSF-1 ELISA Kit; BSF1 ELISA Kit; IGG1 induction factor ELISA Kit; IL 4 ELISA Kit; IL-4 ELISA Kit; IL4 ELISA Kit; IL4_HUMAN ELISA Kit; Il4e12 ELISA Kit; Interleukin 4 ELISA Kit; Interleukin 4 variant 2 ELISA Kit; Interleukin 4; isoform 1 ELISA Kit; Interleukin-4 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit; MGC79402 ELISA Kit; Pitrakinra ELISA Kit
Abbreviation IL4
Species Homo sapiens (Human)
Sample Types serum, tissue homogenates, cell culture supernates, saliva
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%
Three samples of known concentration were tested in twenty assays to assess. 
 

 
Linearity To assess the linearity of the assay, samples were spiked with high concentrations of human IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Recovery The recovery of human IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Typical Data These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IL-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Squirt bottle, manifold dispenser, or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100ml and 500ml graduated cylinders.
  • Deionized or distilled water.
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
  • Timer
  • Test tubes for dilution.
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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