Human Interleukin 5,IL-5 ELISA KIT
Product Details
| Target Name |
interleukin 5 (colony-stimulating factor, eosinophil) |
| Alternative Names |
B-cell differentiation factor I ELISA Kit; Colony stimulating factor ELISA Kit; EDF ELISA Kit; Eosinophil differentiation factor ELISA Kit; IL-5 ELISA Kit; IL5 ELISA Kit; IL5_HUMAN ELISA Kit; Interleukin 5 (colony stimulating factor; eosinophil) ELISA Kit; Interleukin 5 ELISA Kit; Interleukin-5 ELISA Kit; T Cell Replacing Factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit |
| Abbreviation |
IL5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 pg/mL-50 pg/mL |
| Sensitivity |
0.195 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
87-93 |
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| 1:2 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-97 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-102 |
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| Recovery |
| The recovery of human IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
92-98 |
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| EDTA plasma (n=4) |
97 |
94-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.771 |
2.745 |
2.758 |
2.630 |
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| 25 |
2.205 |
2.187 |
2.196 |
2.068 |
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| 12.5 |
1.352 |
1.374 |
1.363 |
1.235 |
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| 6.25 |
0.701 |
0.710 |
0.706 |
0.578 |
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| 3.12 |
0.424 |
0.432 |
0.428 |
0.300 |
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| 1.56 |
0.301 |
0.288 |
0.295 |
0.167 |
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| 0.78 |
0.214 |
0.206 |
0.210 |
0.082 |
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| 0 |
0.129 |
0.127 |
0.128 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
