Human Interleukin17B (IL17B) ELISA Kit
Product Details
| Target Name |
interleukin 17B |
| Alternative Names |
Cytokine like protein Zcyto7 ELISA Kit; Cytokine Zcyto7 ELISA Kit; IL 17B ELISA Kit; IL 20 ELISA Kit; IL-17B ELISA Kit; IL-20 ELISA Kit; IL17B ELISA Kit; IL17B_HUMAN ELISA Kit; IL20 ELISA Kit; Interleukin 17 beta ELISA Kit; Interleukin 17B ELISA Kit; Interleukin 20 ELISA Kit; Interleukin-17B ELISA Kit; Interleukin-20 ELISA Kit; MGC138900 ELISA Kit; MGC138901 ELISA Kit; Neuronal interleukin 17 related factor ELISA Kit; Neuronal interleukin-17-related factor ELISA Kit; NIRF ELISA Kit; ZCYTO7 ELISA Kit |
| Abbreviation |
IL17B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL17B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
106 |
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| Range % |
103-109 |
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| 1:2 |
Average % |
93 |
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| Range % |
92-98 |
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| 1:4 |
Average % |
92 |
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| Range % |
86-96 |
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| 1:8 |
Average % |
94 |
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| Range % |
89-97 |
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| Recovery |
| The recovery of human IL17B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
103 |
99-109 |
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| EDTA plasma (n=4) |
92 |
87-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.066 |
2.122 |
2.094 |
1.974 |
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| 400 |
1.574 |
1.625 |
1.600 |
1.480 |
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| 200 |
1.082 |
1.113 |
1.098 |
0.978 |
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| 100 |
0.643 |
0.687 |
0.665 |
0.545 |
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| 50 |
0.391 |
0.406 |
0.399 |
0.279 |
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| 25 |
0.256 |
0.255 |
0.256 |
0.136 |
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| 12.5 |
0.211 |
0.221 |
0.216 |
0.096 |
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| 0 |
0.119 |
0.121 |
0.120 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
