Human L-Selectin ELISA kit
Product Details
| Target Name |
selectin L |
| Alternative Names |
A.11 ELISA Kit; AI528707 ELISA Kit; CD62 antigen ligand ELISA Kit; CD62 antigen-like family member L ELISA Kit; CD62L ELISA Kit; gp90-MEL ELISA Kit; IgA nephropathy, susceptibility to, included ELISA Kit; L Selectin ELISA Kit; L-selectin ELISA Kit; LAM-1 ELISA Kit; LAM1 ELISA Kit; LECAM1 ELISA Kit; LEU8 ELISA Kit; Leukocyte adhesion molecule 1 ELISA Kit; Leukocyte surface antigen Leu-8 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 1 ELISA Kit; Lnhr ELISA Kit; LSEL ELISA Kit; Ly-22 ELISA Kit; Ly-m22 ELISA Kit; Lyam-1 ELISA Kit; LYAM1 ELISA Kit; LYAM1_HUMAN ELISA Kit; Lymph node homing receptor ELISA Kit; Lymphocyte adhesion molecule 1 ELISA Kit; Lymphocyte antigen 22 ELISA Kit; Lymphocyte surface MEL-14 antigen ELISA Kit; MEL-14 ELISA Kit; Pln homing receptor ELISA Kit; PLNHR ELISA Kit; Selectin L ELISA Kit; Selectin, lymphocyte ELISA Kit; SELL ELISA Kit; TQ1 ELISA Kit |
| Abbreviation |
SELL |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Stem Cells |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human L-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
90 |
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| Range % |
84-95 |
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| 1:10 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:20 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:40 |
Average % |
94 |
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| Range % |
86-101 |
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| Recovery |
| The recovery of human L-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
86-94 |
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| EDTA plasma (n=4) |
92 |
85-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.498 |
2.662 |
2.580 |
2.409 |
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| 25 |
2.166 |
2.252 |
2.209 |
2.038 |
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| 12.5 |
1.651 |
1.669 |
1.660 |
1.489 |
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| 6.25 |
0.932 |
0.967 |
0.950 |
0.779 |
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| 3.12 |
0.550 |
0.514 |
0.532 |
0.361 |
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| 1.56 |
0.363 |
0.352 |
0.358 |
0.187 |
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| 0.78 |
0.265 |
0.284 |
0.275 |
0.104 |
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| 0 |
0.173 |
0.169 |
0.171 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
