Human Laminin-5(LN-5)ELISA Kit
Product Details
| Target Name |
laminin, alpha 5 |
| Alternative Names |
KIAA0533 ELISA Kit; KIAA1907 ELISA Kit; LAMA5 ELISA Kit; LAMA5_HUMAN ELISA Kit; Laminin alpha 5 chain ELISA Kit; Laminin subunit alpha 5 ELISA Kit; Laminin subunit alpha-5 ELISA Kit; Laminin; alpha 5 ELISA Kit; Laminin-10 subunit alpha ELISA Kit; Laminin-11 subunit alpha ELISA Kit; Laminin-15 subunit alpha ELISA Kit; RP11-157P1.6 ELISA Kit |
| Abbreviation |
LAMA5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
12.5 ng/mL-800 ng/mL |
| Sensitivity |
3.12 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of Human LN-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
84-94 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
102 |
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| Range % |
96-106 |
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| Recovery |
| The recovery of Human LN-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
88-98 |
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| EDTA plasma (n=4) |
86 |
82-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.262 |
2.368 |
2.315 |
2.216 |
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| 400 |
1.676 |
1.570 |
1.623 |
1.524 |
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| 200 |
0.987 |
0.960 |
0.974 |
0.875 |
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| 100 |
0.558 |
0.547 |
0.553 |
0.454 |
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| 50 |
0.312 |
0.324 |
0.318 |
0.219 |
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| 25 |
0.243 |
0.256 |
0.250 |
0.151 |
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| 12.5 |
0.172 |
0.174 |
0.173 |
0.074 |
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| 0 |
0.098 |
0.099 |
0.099 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
