Human lectin-galactose binding-soluble 3,Lgals3 ELISA Kit
Product Details
| Target Name |
lectin, galactoside-binding, soluble, 3 |
| Alternative Names |
35 kDa lectin ELISA Kit; Carbohydrate binding protein 35 ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; CBP35 ELISA Kit; Gal-3 ELISA Kit; GAL3 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; Galactoside-binding protein ELISA Kit; GALBP ELISA Kit; Galectin 3 internal gene,included ELISA Kit; Galectin-3 ELISA Kit; Galectin3 ELISA Kit; GALIG ELISA Kit; GBP ELISA Kit; IgE binding protein ELISA Kit; IgE-binding protein ELISA Kit; L 31 ELISA Kit; L 34 ELISA Kit; L-31 ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; L31 ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Lectin, galactose binding, soluble 3 ELISA Kit; LEG3_HUMAN ELISA Kit; LGALS2 ELISA Kit; LGALS3 ELISA Kit; MAC 2 antigen ELISA Kit; Mac-2 ELISA Kit; Mac-2 antigen ELISA Kit; MAC2 ELISA Kit; Macrophage galactose-specific lectin ELISA Kit; MGC105387 ELISA Kit |
| Abbreviation |
LGALS3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, saliva |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Lgals3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-95 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
93 |
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| Range % |
88-100 |
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| Recovery |
| The recovery of human Lgals3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
86-97 |
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| EDTA plasma (n=4) |
102 |
94-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.346 |
2.331 |
2.339 |
2.185 |
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| 20 |
1.659 |
1.676 |
1.668 |
1.514 |
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| 10 |
1.139 |
1.151 |
1.145 |
0.991 |
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| 5 |
0.699 |
0.678 |
0.689 |
0.535 |
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| 2.5 |
0.498 |
0.487 |
0.493 |
0.339 |
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| 1.25 |
0.336 |
0.343 |
0.340 |
0.186 |
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| 0.625 |
0.227 |
0.212 |
0.220 |
0.066 |
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| 0 |
0.156 |
0.152 |
0.154 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
