Human Lipopolysaccharides,LPS ELISA Kit
Product Details
| Description |
The human lipopolysaccharides/LPS ELISA kit is a ready-to-use microwell, strip plate ELISA kit for the quantitative measurement of human LPS in biological samples, including serum, plasma, and tissue homogenates. It employs the sandwich ELISA technique in the detection of target analytes in the samples. This ELISA kit has been quality controlled in multiple characteristics, including sensitivity, specificity, precision, linearity, and recovery. See the product instructions to obtain more information.
LPS, a major component of Gram-negative bacteria, is a pathogen-associated molecular pattern (PAMP) molecule that mediates the bacterial infection. It also plays an important role in endotoxic shock. LPS-TLR4-MD2 interaction activates the MYD88-dependent pathway that leads to inflammation and bacterial clearance and the MYD88-independent pathway critical for adjuvanticity.
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| Target Name |
Lipopolysaccharides,LPS |
| Abbreviation |
LPS |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
94 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-97 |
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| EDTA plasma (n=4) |
88 |
82-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.809 |
2.960 |
2.885 |
2.720 |
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| 200 |
2.227 |
2.366 |
2.297 |
2.132 |
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| 100 |
1.515 |
1.556 |
1.536 |
1.371 |
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| 50 |
0.789 |
0.755 |
0.772 |
0.607 |
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| 25 |
0.432 |
0.470 |
0.451 |
0.286 |
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| 12.5 |
0.303 |
0.325 |
0.314 |
0.149 |
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| 6.25 |
0.235 |
0.232 |
0.234 |
0.069 |
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| 0 |
0.164 |
0.165 |
0.165 |
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| Materials provided |
- An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
- Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
- Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
- Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
- HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
- Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
- TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
- Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
