Human Long palate, lung and nasal epithelium carcinoma-associated protein 4(C20orf186) ELISA kit
Product Details
| Target Name |
chromosome 20 open reading frame 186 |
| Alternative Names |
BPI fold-containing family B member 4 ELISA Kit; Bpifb4 ELISA Kit; C20orf186 ELISA Kit; dJ726C3.5 ELISA Kit; Gm1006 ELISA Kit; Ligand-binding protein RY2G5 ELISA Kit; Long palate; lung and nasal epithelium carcinoma-associated protein 4 ELISA Kit; LPLC4_HUMAN ELISA Kit; Lplunc4 ELISA Kit |
| Abbreviation |
C20orf186 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
25 pg/mL-1600 pg/mL |
| Sensitivity |
6.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human C20orf186 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
86-97 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
92-106 |
|
| 1:4 |
Average % |
84 |
|
| Range % |
81-89 |
|
| 1:8 |
Average % |
91 |
|
| Range % |
83-98 |
|
|
| Recovery |
| The recovery of human C20orf186 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
101 |
96-105 |
|
| EDTA plasma (n=4) |
93 |
88-99 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1600 |
2.908 |
3.158 |
3.033 |
2.897 |
|
| 800 |
2.304 |
2.433 |
2.369 |
2.233 |
|
| 400 |
1.478 |
1.475 |
1.477 |
1.341 |
|
| 200 |
0.686 |
0.707 |
0.697 |
0.561 |
|
| 100 |
0.497 |
0.503 |
0.500 |
0.364 |
|
| 50 |
0.263 |
0.277 |
0.270 |
0.134 |
|
| 25 |
0.230 |
0.244 |
0.237 |
0.101 |
|
| 0 |
0.140 |
0.132 |
0.136 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
