Human low-density lipoprotein-receptor-related protein 5,LRP-5 ELISA Kit
Product Details
| Target Name |
low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor) |
| Alternative Names |
A2MR ELISA Kit; Alpha 2 macroglobulin receptor ELISA Kit; alpha 2MR ELISA Kit; Alpha-2-macroglobulin receptor ELISA Kit; APOER ELISA Kit; Apolipoprotein E receptor ELISA Kit; APR ELISA Kit; CD 91 ELISA Kit; CD91 ELISA Kit; CD91 antigen ELISA Kit; IGFBP3R ELISA Kit; LDL receptor related protein 1 ELISA Kit; Low density lipoprotein receptor related protein 1 ELISA Kit; Low density lipoprotein related protein 1 ELISA Kit; Low-density lipoprotein receptor-related protein 1 intracellular domain ELISA Kit; LRP 1 ELISA Kit; LRP 515 ELISA Kit; LRP 85 ELISA Kit; LRP ELISA Kit; LRP ICD ELISA Kit; LRP-1 ELISA Kit; LRP-515 ELISA Kit; LRP-85 ELISA Kit; Lrp1 ELISA Kit; LRP1 protein ELISA Kit; LRP1_HUMAN ELISA Kit; LRP1A ELISA Kit; LRP515 ELISA Kit; LRP85 ELISA Kit; LRPICD ELISA Kit; MGC88725 ELISA Kit; Prolow density lipoprotein receptor related protein 1 ELISA Kit; TbetaR V/LRP 1/IGFBP 3 receptor ELISA Kit; TbetaRV/LRP1/IGFBP3 receptor ELISA Kit; TGFBR 5 ELISA Kit; TGFBR5 ELISA Kit; Type V tgf beta receptor ELISA Kit |
| Abbreviation |
LRP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human LRP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
88-97 |
|
| 1:2 |
Average % |
84 |
|
| Range % |
80-89 |
|
| 1:4 |
Average % |
102 |
|
| Range % |
96-108 |
|
| 1:8 |
Average % |
97 |
|
| Range % |
91-102 |
|
|
| Recovery |
| The recovery of human LRP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
86 |
82-94 |
|
| EDTA plasma (n=4) |
99 |
92-107 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
2.455 |
2.354 |
2.405 |
2.262 |
|
| 20 |
1.628 |
1.527 |
1.578 |
1.435 |
|
| 10 |
0.976 |
0.955 |
0.966 |
0.823 |
|
| 5 |
0.566 |
0.577 |
0.572 |
0.429 |
|
| 2.5 |
0.371 |
0.382 |
0.377 |
0.234 |
|
| 1.25 |
0.267 |
0.278 |
0.273 |
0.130 |
|
| 0.625 |
0.210 |
0.216 |
0.213 |
0.070 |
|
| 0 |
0.142 |
0.143 |
0.143 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
