Human low-density lipoprotein-receptor-related protein,LRP-6 ELISA Kit
Product Details
| Target Name |
low density lipoprotein receptor-related protein 6 |
| Alternative Names |
ADCAD2 ELISA Kit; C030016K15Rik ELISA Kit; Cd ELISA Kit; FLJ90062 ELISA Kit; FLJ90421 ELISA Kit; LDL receptor related protein 6 ELISA Kit; Low density lipoprotein receptor related protein 6 ELISA Kit; Low-density lipoprotein receptor-related protein 6 ELISA Kit; LRP-6 ELISA Kit; LRP6 ELISA Kit; LRP6_HUMAN ELISA Kit; OTTHUMP00000238979 ELISA Kit; OTTHUMP00000238980 ELISA Kit; OTTHUMP00000238982 ELISA Kit; STHAG7 ELISA Kit |
| Abbreviation |
LRP6 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, tissue homogenates, cell lysates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of Human LRP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
88 |
|
| Range % |
83-91 |
|
| 1:2 |
Average % |
92 |
|
| Range % |
86-97 |
|
| 1:4 |
Average % |
94 |
|
| Range % |
88-98 |
|
| 1:8 |
Average % |
102 |
|
| Range % |
98-104 |
|
|
| Recovery |
| The recovery of Human LRP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
88-98 |
|
| EDTA plasma (n=4) |
86 |
82-92 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.625 |
2.729 |
2.677 |
2.494 |
|
| 10 |
2.146 |
2.047 |
2.097 |
1.914 |
|
| 5 |
1.444 |
1.463 |
1.454 |
1.271 |
|
| 2.5 |
0.912 |
0.928 |
0.920 |
0.737 |
|
| 1.25 |
0.597 |
0.586 |
0.592 |
0.409 |
|
| 0.625 |
0.401 |
0.416 |
0.409 |
0.226 |
|
| 0.312 |
0.287 |
0.275 |
0.281 |
0.098 |
|
| 0 |
0.182 |
0.183 |
0.183 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
