Human lumican,LUM ELISA Kit
Product Details
| Target Name |
lumican |
| Alternative Names |
Keratan sulfate proteoglycan lumican ELISA Kit; KSPG lumican ELISA Kit; LDC ELISA Kit; LUM ELISA Kit; LUM_HUMAN ELISA Kit; Lumican ELISA Kit; Lumican precursor ELISA Kit; SLRR2D ELISA Kit |
| Abbreviation |
LUM |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, urine, cell culture supernates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human LUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
85-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
100 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human LUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-110 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.858 |
2.889 |
2.874 |
2.743 |
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| 20 |
2.361 |
2.381 |
2.371 |
2.240 |
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| 10 |
1.752 |
1.852 |
1.802 |
1.671 |
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| 5 |
1.134 |
1.233 |
1.184 |
1.053 |
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| 2.5 |
0.653 |
0.695 |
0.674 |
0.543 |
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| 1.25 |
0.431 |
0.423 |
0.427 |
0.296 |
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| 0.625 |
0.296 |
0.321 |
0.309 |
0.178 |
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| 0 |
0.133 |
0.129 |
0.131 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
