Human lymphocyte function associated antigen 3,LFA-3 ELISA Kit
Product Details
| Target Name |
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| Alternative Names |
95 subunit beta ELISA Kit; Beta 2 (antigen CD18 (p95) ELISA Kit; CD 18 ELISA Kit; CD18 ELISA Kit; Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) ELISA Kit; Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta ELISA Kit; Cell surface adhesion glycoproteins LFA-1/CR3/p150 ELISA Kit; Complement component 3 receptor 3 and 4 subunit ELISA Kit; Complement receptor C3 beta subunit ELISA Kit; Complement receptor C3 subunit beta ELISA Kit; Integrin beta 2 ELISA Kit; Integrin beta chain beta 2 ELISA Kit; Integrin beta-2 (ITGB2) ELISA Kit; Integrin beta-2 ELISA Kit; Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) ELISA Kit; ITB2_HUMAN ELISA Kit; ITGB2 ELISA Kit; LAD ELISA Kit; LCAMB ELISA Kit; Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C ELISA Kit; Leukocyte cell adhesion molecule CD18 ELISA Kit; LFA 1 ELISA Kit; LFA1 ELISA Kit; Lymphocyte function associated antigen 1 (LFA1) ELISA Kit; Lymphocyte function associated antigen 1 ELISA Kit; MAC 1 ELISA Kit; MAC1 ELISA Kit; Macrophage antigen 1 (mac1) beta ELISA Kit; MF17 ELISA Kit; MFI7 ELISA Kit; OTTHUMP00000115278 ELISA Kit; OTTHUMP00000115279 ELISA Kit; OTTHUMP00000115280 ELISA Kit; OTTHUMP00000115281 ELISA Kit; OTTHUMP00000115282 ELISA Kit |
| Abbreviation |
ITGB2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITG β2/CD11+CD18CD18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
91 |
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| Range % |
84-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-98 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-102 |
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| Recovery |
| The recovery of human ITG β2/CD11+CD18CD18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
96 |
87-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
1.631 |
1.609 |
1.620 |
1.523 |
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| 500 |
1.365 |
1.318 |
1.342 |
1.245 |
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| 250 |
0.952 |
0.943 |
0.948 |
0.851 |
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| 125 |
0.650 |
0.635 |
0.643 |
0.546 |
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| 62.5 |
0.381 |
0.377 |
0.379 |
0.282 |
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| 31.2 |
0.267 |
0.259 |
0.263 |
0.166 |
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| 15.6 |
0.185 |
0.191 |
0.188 |
0.091 |
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| 0 |
0.095 |
0.099 |
0.097 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
