Human mannan-binding lectin serine peptidase 2 (MASP2)ELISA kit
Product Details
| Target Name |
mannan-binding lectin serine peptidase 2 |
| Alternative Names |
MASP2Mannan-binding lectin serine protease 2 ELISA Kit; EC 3.4.21.104 ELISA Kit; MBL-associated serine protease 2 ELISA Kit; Mannose-binding protein-associated serine protease 2 ELISA Kit; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain] ELISA Kit |
| Abbreviation |
MASP2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, cerebrospinal fluid (CSF) |
| Detection Range |
23.44 pg/mL-1500 pg/mL |
| Sensitivity |
5.86 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
101 |
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| Range % |
91-111 |
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| 1:400 |
Average % |
97 |
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| Range % |
91-108 |
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| 1:800 |
Average % |
99 |
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| Range % |
92-106 |
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| 1:1600 |
Average % |
95 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human MASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
85-99 |
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| EDTA plasma (n=4) |
96 |
90-112 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
2.540 |
2.539 |
2.540 |
2.395 |
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| 750 |
2.022 |
2.027 |
2.025 |
1.880 |
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| 375 |
1.154 |
1.190 |
1.172 |
1.027 |
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| 187.5 |
0.702 |
0.760 |
0.731 |
0.586 |
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| 93.75 |
0.380 |
0.391 |
0.386 |
0.241 |
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| 46.88 |
0.280 |
0.300 |
0.290 |
0.145 |
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| 23.44 |
0.175 |
0.161 |
0.168 |
0.023 |
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| 0 |
0.140 |
0.150 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
