Human Mannma binding protein/mannan binding lectin,MBP/MBL ELISA Kit
Product Details
| Target Name |
mannose-binding lectin (protein C) 2, soluble (opsonic defect) |
| Alternative Names |
COLEC 1 ELISA Kit; COLEC1 ELISA Kit; Collectin-1 ELISA Kit; HSMBPC ELISA Kit; Lectin; mannose-binding; soluble; 2 ELISA Kit; Mannan binding lectin ELISA Kit; Mannan binding protein ELISA Kit; Mannan-binding protein ELISA Kit; Mannose binding lectin (protein C) 2 soluble ELISA Kit; Mannose binding lectin (protein C) 2; soluble (opsonic defect) ELISA Kit; Mannose binding lectin (protein C) 2; soluble ELISA Kit; Mannose binding lectin 2 soluble ELISA Kit; Mannose binding lectin 2; soluble (opsonic defect) ELISA Kit; Mannose binding lectin ELISA Kit; Mannose binding lectin protein C2 soluble opsonic defect ELISA Kit; Mannose binding protein ELISA Kit; Mannose binding protein C ELISA Kit; Mannose binding protein C precursor ELISA Kit; Mannose binding protein; serum ELISA Kit; Mannose-binding lectin ELISA Kit; Mannose-binding protein C ELISA Kit; MBL 2 ELISA Kit; MBL ELISA Kit; MBL2 ELISA Kit; MBL2_HUMAN ELISA Kit; MBL2D ELISA Kit; MBP 1 ELISA Kit; MBP ELISA Kit; MBP C ELISA Kit; MBP-C ELISA Kit; MBP1 ELISA Kit; MBPB ELISA Kit; MBPC ELISA Kit; MBPD ELISA Kit; MGC116832 ELISA Kit; MGC116833 ELISA Kit; Opsonic defect ELISA Kit; protein C ELISA Kit; Soluble mannose binding lectin ELISA Kit |
| Abbreviation |
MBL2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum |
| Detection Range |
1.563 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MBL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
97 |
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| Range % |
80-106 |
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| 1:200 |
Average % |
93 |
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| Range % |
91-95 |
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| 1:400 |
Average % |
93 |
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| Range % |
85-96 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-101 |
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| Recovery |
| The recovery of human MBL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
1.902 |
1.945 |
1.924 |
1.838 |
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| 50 |
1.720 |
1.596 |
1.658 |
1.572 |
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| 25 |
1.245 |
1.350 |
1.298 |
1.212 |
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| 12.5 |
0.830 |
0.885 |
0.858 |
0.772 |
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| 6.25 |
0.619 |
0.628 |
0.624 |
0.538 |
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| 3.12 |
0.380 |
0.388 |
0.384 |
0.298 |
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| 1.56 |
0.268 |
0.243 |
0.256 |
0.170 |
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| 0 |
0.087 |
0.085 |
0.086 |
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