Human Matrix Gla Protein,MGP ELISA Kit
Product Details
| Description |
MGP is a small secretary protein primarily secreted by vascular smooth muscle cells (VSMCs) in the arterial wall. As a potent inhibitor of vascular calcification (VC), MGP exerts calcification inhibition through vitamin K-dependent carboxylation. Additionally, MGP is also involved in fat metabolism and serum inactive MGP level is related to visceral fat.
This assay employs the quantitative sandwich enzyme immunoassay technique. Standards and samples are pipetted into the wells and any MGP present is bound by the anti-human MGP antibody pre-coated onto the microplate. After removing any unbound substances, a biotin-conjugated MGP antibody is added to the wells. After washing, HRP-avidin conjugate is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of MGP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| Target Name |
matrix Gla protein |
| Alternative Names |
Cell growth inhibiting gene 36 protein ELISA Kit; Cell growth-inhibiting gene 36 protein ELISA Kit; GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX ELISA Kit; GIG36 ELISA Kit; Matrix Gla protein ELISA Kit; MGLAP ELISA Kit; MGP ELISA Kit; MGP_HUMAN ELISA Kit; NTI ELISA Kit |
| Abbreviation |
MGP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
7.8 pg/mL-500 pg/mL |
| Sensitivity |
1.95 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
100 |
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| Range % |
95-115 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-111 |
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| 1:400 |
Average % |
97 |
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| Range % |
92-106 |
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| 1:800 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
101 |
96-110 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.712 |
2.778 |
2.745 |
2.641 |
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| 250 |
2.206 |
2.231 |
2.219 |
2.115 |
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| 125 |
1.507 |
1.537 |
1.522 |
1.418 |
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| 62.5 |
0.895 |
0.904 |
0.900 |
0.796 |
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| 31.2 |
0.457 |
0.496 |
0.477 |
0.373 |
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| 15.6 |
0.254 |
0.289 |
0.272 |
0.168 |
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| 7.8 |
0.162 |
0.169 |
0.166 |
0.062 |
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| 0 |
0.101 |
0.106 |
0.104 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human MGP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled MGP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent(50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
