Human Matrix Metalloproteinase 12(MMP-12) ELISA Kit
Product Details
| Target Name |
matrix metallopeptidase 12 (macrophage elastase) |
| Alternative Names |
EC 3.4.24.65 ELISA Kit; HME ELISA Kit; Macrophage elastase ELISA Kit; Macrophage metalloelastase ELISA Kit; Macrophage metaloelastase ELISA Kit; Matrix metallopeptidase 12 (macrophage elastase) ELISA Kit; Matrix metalloprotease 12 ELISA Kit; Matrix metalloproteinase-12 ELISA Kit; ME ELISA Kit; MGC138506 ELISA Kit; MME ELISA Kit; MMP 12 ELISA Kit; MMP-12 ELISA Kit; Mmp12 ELISA Kit; MMP12_HUMAN ELISA Kit |
| Abbreviation |
MMP12 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:2 |
Average % |
89 |
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| Range % |
81-96 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-106 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of human MMP-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
88-98 |
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| EDTA plasma (n=4) |
97 |
92-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.695 |
2.594 |
2.645 |
2.514 |
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| 20 |
2.132 |
2.031 |
2.082 |
1.951 |
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| 10 |
1.423 |
1.444 |
1.434 |
1.303 |
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| 5 |
0.813 |
0.802 |
0.808 |
0.677 |
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| 2.5 |
0.455 |
0.445 |
0.450 |
0.319 |
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| 1.25 |
0.290 |
0.301 |
0.296 |
0.165 |
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| 0.625 |
0.220 |
0.214 |
0.217 |
0.086 |
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| 0 |
0.131 |
0.130 |
0.131 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
