Human Melanoma-associated antigen D2(MAGED2) ELISA kit
Product Details
| Target Name |
melanoma antigen family D, 2 |
| Alternative Names |
11B6 ELISA Kit; BCG 1 ELISA Kit; BCG-1 ELISA Kit; Breast cancer-associated gene 1 protein ELISA Kit; HCA10 ELISA Kit; Hepatocellular carcinoma-associated protein JCL-1 ELISA Kit; JCL 1 ELISA Kit; MAGD2_HUMAN ELISA Kit; MAGE D ELISA Kit; MAGE D2 antigen ELISA Kit; MAGE-D2 antigen ELISA Kit; MAGED2 ELISA Kit; Melanoma antigen family D 2 ELISA Kit; Melanoma associated antigen D2 ELISA Kit; Melanoma-associated antigen D2 ELISA Kit |
| Abbreviation |
MAGED2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
83-94 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:8 |
Average % |
107 |
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| Range % |
102-111 |
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| Recovery |
| The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
105 |
102-108 |
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| EDTA plasma (n=4) |
96 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.238 |
2.334 |
2.286 |
2.090 |
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| 1000 |
1.415 |
1.527 |
1.471 |
1.275 |
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| 500 |
0.881 |
0.932 |
0.907 |
0.711 |
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| 250 |
0.560 |
0.573 |
0.567 |
0.371 |
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| 125 |
0.379 |
0.381 |
0.380 |
0.184 |
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| 62.5 |
0.292 |
0.284 |
0.288 |
0.092 |
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| 31.25 |
0.247 |
0.252 |
0.250 |
0.054 |
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| 0 |
0.195 |
0.196 |
0.196 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
