Human Mesothelin(MSLN)ELISA KIT
Product Details
| Alternative Names |
CAK 1 ELISA Kit; CAK1 ELISA Kit; CAK1 antigen ELISA Kit; cleaved form ELISA Kit; Megakaryocyte potentiating factor ELISA Kit; Mesothelin ELISA Kit; Mesothelin isoform 1 precursor ELISA Kit; MPF ELISA Kit; Msln ELISA Kit; MSLN_HUMAN ELISA Kit; Pre pro megakaryocyte potentiating factor ELISA Kit; Pre-pro-megakaryocyte-potentiating factor ELISA Kit; SMR ELISA Kit; SMRP ELISA Kit; Soluble MPF mesothelin related protein ELISA Kit |
| Abbreviation |
MSLN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8> |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10> |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MSLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
87 |
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| Range % |
83-92 |
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| 1:10 |
Average % |
101 |
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| Range % |
96-104 |
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| 1:20 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:40 |
Average % |
86 |
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| Range % |
81-92 |
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| Recovery |
| The recovery of human MSLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
88-97 |
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| EDTA plasma (n=4) |
102 |
96-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.875 |
2.956 |
2.916 |
2.826 |
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| 10 |
2.246 |
2.386 |
2.316 |
2.226 |
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| 5 |
1.512 |
1.544 |
1.528 |
1.438 |
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| 2.5 |
0.808 |
0.815 |
0.812 |
0.722 |
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| 1.25 |
0.455 |
0.447 |
0.451 |
0.361 |
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| 0.625 |
0.246 |
0.253 |
0.250 |
0.160 |
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| 0.312 |
0.171 |
0.176 |
0.174 |
0.084 |
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| 0 |
0.089 |
0.090 |
0.090 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
