The Human Metallothionein (MT) ELISA kit is specifically designed and validated to quantitatively detect the levels of MT in multiple biological solutions, including serum, plasma, cell culture supernates, tissue homogenates, urine, or saliva. It is not intended for diagnostic use. This assay kit was designed and optimized for epigenetics and nuclear signaling research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay is based on the Sandwich-ELISA mechanism (the MT in the sample is sandwiched between the pre-coated MT antibody and Biotin-labeled MT antibody) and enzyme-substrate chromogenic reaction. The color intensity is positively related to the amount of MT bound in the initial step. The levels of MT in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

MTs are a group of small cysteine-rich cytosolic proteins involved in metal homeostasis and detoxification of heavy metals, DNA damage, and oxidative stress. Recently, many studies have demonstrated that MTs are crucial participants in tumor growth, differentiation, angiogenesis, metastasis, microenvironment remodeling, immune escape, and drug resistance.