Human Metastasis-suppressor KiSS-1(KISS1) ELISA kit
Product Details
| Target Name |
KiSS-1 metastasis-suppressor |
| Alternative Names |
KISS 1 ELISA Kit; KiSS 1 metastasis suppressor ELISA Kit; Kiss1 ELISA Kit; KISS1_HUMAN ELISA Kit; Kisspeptin 1 ELISA Kit; Kisspeptin 10 ELISA Kit; Kisspeptin 13 ELISA Kit; Kisspeptin 14 ELISA Kit; Kisspeptin ELISA Kit; Kisspeptin-1 ELISA Kit; Kisspeptin-10 ELISA Kit; Kisspeptin-54 ELISA Kit; Kisspeptin1 ELISA Kit; Malignant melanoma metastasis suppressor ELISA Kit; Metastasis suppressor KiSS 1 ELISA Kit; Metastasis suppressor KiSS1 ELISA Kit; Metastin ELISA Kit; MGC39258 ELISA Kit |
| Abbreviation |
KISS1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-94 |
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| 1:2 |
Average % |
101 |
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| Range % |
97-103 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
95 |
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| Range % |
91-100 |
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| Recovery |
| The recovery of human KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
80-93 |
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| EDTA plasma (n=4) |
96 |
92-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.842 |
2.963 |
2.903 |
2.775 |
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| 10 |
2.187 |
2.203 |
2.195 |
2.067 |
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| 5 |
1.148 |
1.226 |
1.187 |
1.059 |
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| 2.5 |
0.755 |
0.743 |
0.749 |
0.621 |
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| 1.25 |
0.349 |
0.365 |
0.357 |
0.229 |
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| 0.625 |
0.222 |
0.205 |
0.214 |
0.086 |
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| 0.312 |
0.202 |
0.200 |
0.201 |
0.073 |
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| 0 |
0.132 |
0.124 |
0.128 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
