Human Mitochondrial brown fat uncoupling protein 1(UCP1) ELISA kit
Product Details
| Target Name |
uncoupling protein 1 (mitochondrial, proton carrier) |
| Alternative Names |
mitochondrial brown fat uncoupling protein ELISA Kit; Mitochondrial brown fat uncoupling protein 1 ELISA Kit; SLC25A7 ELISA Kit; Solute carrier family 25 member 7 ELISA Kit; Thermogenin ELISA Kit; UCP 1 ELISA Kit; UCP ELISA Kit; UCP1 ELISA Kit; UCP1_HUMAN ELISA Kit; uncoupling protein 1 (mitochondrial, proton carrier) ELISA Kit; Uncoupling protein 1 ELISA Kit |
| Abbreviation |
UCP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-99 |
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| 1:2 |
Average % |
101 |
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| Range % |
93-105 |
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| 1:4 |
Average % |
94 |
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| Range % |
86-98 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-105 |
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| Recovery |
| The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-98 |
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| EDTA plasma (n=4) |
92 |
89-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.093 |
1.952 |
2.023 |
1.940 |
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| 1000 |
1.344 |
1.321 |
1.333 |
1.250 |
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| 500 |
0.781 |
0.777 |
0.779 |
0.696 |
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| 250 |
0.471 |
0.455 |
0.463 |
0.380 |
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| 125 |
0.283 |
0.278 |
0.281 |
0.198 |
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| 62.5 |
0.212 |
0.207 |
0.210 |
0.127 |
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| 31.25 |
0.149 |
0.145 |
0.147 |
0.064 |
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| 0 |
0.085 |
0.081 |
0.083 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
