Human mucin-5 subtype B,MUC5B ELISA kit
Product Details
| Target Name |
mucin 5B, oligomeric mucus/gel-forming |
| Alternative Names |
2300002I04Rik ELISA Kit; A130042M24 ELISA Kit; AV085033 ELISA Kit; Cervical mucin ELISA Kit; Cervical mucin MUC5B ELISA Kit; High molecular weight salivary mucin MG1 ELISA Kit; MG1 ELISA Kit; MUC-5B ELISA Kit; MUC5 ELISA Kit; MUC5B ELISA Kit; MUC5B_HUMAN ELISA Kit; MUC9 ELISA Kit; Mucin 5 subtype B, tracheobronchial ELISA Kit; Mucin 5B ELISA Kit; Mucin 5B oligomeric mucus/gel forming ELISA Kit; Mucin-5 subtype B ELISA Kit; Mucin-5B ELISA Kit; Sublingual gland mucin ELISA Kit; tracheobronchial ELISA Kit |
| Abbreviation |
MUC5B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
92-101 |
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| 1:2 |
Average % |
99 |
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| Range % |
92-108 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-103 |
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| Recovery |
| The recovery of human MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
92-104 |
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| EDTA plasma (n=4) |
93 |
89-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 500 |
2.508 |
2.607 |
2.558 |
2.415 |
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| 250 |
1.765 |
1.864 |
1.815 |
1.672 |
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| 125 |
1.187 |
1.206 |
1.197 |
1.054 |
|
| 62.5 |
0.791 |
0.801 |
0.796 |
0.653 |
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| 31.2 |
0.447 |
0.438 |
0.443 |
0.300 |
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| 15.6 |
0.302 |
0.311 |
0.307 |
0.164 |
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| 7.8 |
0.238 |
0.232 |
0.235 |
0.092 |
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| 0 |
0.142 |
0.143 |
0.143 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
