Human myelin basic protein,MBP ELISA Kit
Product Details
| Target Name |
myelin basic protein |
| Alternative Names |
GDB ELISA Kit; Golli MBP ELISA Kit; Golli MBP; myelin basic protein ELISA Kit; Hemopoietic MBP ELISA Kit; HMBPR ELISA Kit; HUGO ELISA Kit; MBP ELISA Kit; MBP_CAVPO ELISA Kit; MBP_HUMAN ELISA Kit; MGC99675 ELISA Kit; MLD ELISA Kit; Myelin A1 protein ELISA Kit; Myelin A1 Protein; basic ELISA Kit; Myelin basic protein ELISA Kit; Myelin Deficient ELISA Kit; Myelin membrane encephalitogenic protein ELISA Kit; OTTHUMP00000163776 ELISA Kit; OTTHUMP00000174387 ELISA Kit; OTTHUMP00000174388 ELISA Kit; SHI ELISA Kit; Shiverer ELISA Kit; SP ELISA Kit |
| Abbreviation |
MBP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
89 |
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| Range % |
85-92 |
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| 1:10 |
Average % |
100 |
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| Range % |
97-102 |
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| 1:20 |
Average % |
105 |
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| Range % |
99-108 |
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| 1:40 |
Average % |
92 |
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| Range % |
87-95 |
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| Recovery |
| The recovery of human MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-96 |
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| EDTA plasma (n=4) |
95 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.716 |
2.702 |
2.709 |
2.596 |
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| 5 |
2.393 |
2.384 |
2.389 |
2.276 |
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| 2.5 |
1.766 |
1.888 |
1.827 |
1.714 |
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| 1.25 |
0.968 |
0.948 |
0.958 |
0.845 |
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| 0.625 |
0.672 |
0.681 |
0.677 |
0.564 |
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| 0.312 |
0.393 |
0.387 |
0.390 |
0.277 |
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| 0.156 |
0.257 |
0.245 |
0.251 |
0.138 |
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| 0 |
0.114 |
0.112 |
0.113 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
