Human Myoglobin,MYO/MB ELISA Kit
Product Details
| Target Name |
myoglobin |
| Alternative Names |
MB ELISA Kit; MGC13548 ELISA Kit; MYG_HUMAN ELISA Kit; Myoglobin ELISA Kit |
| Abbreviation |
MB |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYO/MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:10 |
Average % |
92 |
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| Range % |
89-99 |
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| 1:20 |
Average % |
87 |
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| Range % |
83-96 |
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| 1:40 |
Average % |
94 |
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| Range % |
90-100 |
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| 1:80 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human MYO/MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
97 |
90-110 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.175 |
2.211 |
2.193 |
2.107 |
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| 50 |
1.613 |
1.656 |
1.635 |
1.549 |
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| 25 |
1.024 |
1.004 |
1.014 |
0.928 |
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| 12.5 |
0.571 |
0.584 |
0.578 |
0.492 |
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| 6.25 |
0.312 |
0.308 |
0.310 |
0.224 |
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| 3.12 |
0.188 |
0.195 |
0.192 |
0.106 |
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| 1.56 |
0.129 |
0.125 |
0.127 |
0.041 |
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| 0 |
0.085 |
0.087 |
0.086 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
