Human Myosin-11(MYH11) ELISA kit
Product Details
| Target Name |
myosin, heavy chain 11, smooth muscle |
| Alternative Names |
AAT4 ELISA Kit; DKFZp686D10126 ELISA Kit; DKFZp686D19237 ELISA Kit; FAA4 ELISA Kit; FLJ35232 ELISA Kit; MGC126726 ELISA Kit; MGC32963 ELISA Kit; MYH 11 ELISA Kit; MYH11 ELISA Kit; MYH11_HUMAN ELISA Kit; Myosin 11 ELISA Kit; Myosin heavy chain 11 ELISA Kit; Myosin heavy chain 11 smooth muscle ELISA Kit; Myosin heavy chain ELISA Kit; Myosin heavy chain smooth muscle isoform ELISA Kit; Myosin heavy polypeptide 11 smooth muscle ELISA Kit; Myosin-11 ELISA Kit; SMHC ELISA Kit; SMMHC ELISA Kit; smooth muscle isoform ELISA Kit; Smooth muscle myosin heavy chain 11 isoform SM2 ELISA Kit; Smooth muscle myosin heavy chain isoform SM2 ELISA Kit |
| Abbreviation |
MYH11 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
25 pg/mL-1600 pg/mL |
| Sensitivity |
6.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYH11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-101 |
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| 1:8 |
Average % |
95 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human MYH11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
94-101 |
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| EDTA plasma (n=4) |
88 |
82-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1600 |
2.295 |
2.498 |
2.397 |
2.258 |
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| 800 |
1.543 |
1.442 |
1.493 |
1.354 |
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| 400 |
0.891 |
0.843 |
0.867 |
0.728 |
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| 200 |
0.528 |
0.537 |
0.533 |
0.394 |
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| 100 |
0.322 |
0.335 |
0.329 |
0.190 |
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| 50 |
0.255 |
0.267 |
0.261 |
0.122 |
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| 25 |
0.174 |
0.179 |
0.177 |
0.038 |
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| 0 |
0.138 |
0.139 |
0.139 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
