Human NAD (P)H Dehydrogenase, Quinone 1 (NQO1)ELISA Kit
Product Details
| Target Name |
NAD(P)H dehydrogenase, quinone 1 |
| Alternative Names |
Azoreductase ELISA Kit; Cytochrome b 5 reductase ELISA Kit; DHQU ELISA Kit; DIA 4 ELISA Kit; DIA4 ELISA Kit; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) ELISA Kit; Diaphorase (NADH/NADPH) ELISA Kit; Diaphorase 4 ELISA Kit; Dioxin inducible 1 ELISA Kit; DT diaphorase ELISA Kit; DT-diaphorase ELISA Kit; DTD ELISA Kit; Menadione reductase ELISA Kit; NAD(P)H dehydrogenase [quinone] 1 ELISA Kit; NAD(P)H dehydrogenase quinone 1 ELISA Kit; NAD(P)H menadione oxidoreductase 1 dioxin inducible ELISA Kit; NAD(P)H quinone dehydrogenase 1 ELISA Kit; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 ELISA Kit; NAD(P)H:menadione oxidoreductase 1 ELISA Kit; NAD(P)H:Quinone acceptor oxidoreductase type 1 ELISA Kit; NAD(P)H:quinone oxidoreductase 1 ELISA Kit; NAD(P)H:quinone oxireductase ELISA Kit; NMOR 1 ELISA Kit; NMOR I ELISA Kit; NMOR1 ELISA Kit; NMORI ELISA Kit; NQO 1 ELISA Kit; NQO1 ELISA Kit; NQO1_HUMAN ELISA Kit; Phylloquinone reductase ELISA Kit; QR 1 ELISA Kit; QR1 ELISA Kit; Quinone reductase 1 ELISA Kit |
| Abbreviation |
NQO1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
39 pg/mL-2500 pg/mL |
| Sensitivity |
9.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
89-101 |
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| 1:2 |
Average % |
89 |
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| Range % |
91-94 |
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| 1:4 |
Average % |
90 |
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| Range % |
82-94 |
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| 1:8 |
Average % |
94 |
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| Range % |
84-99 |
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| Recovery |
| The recovery of human NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
84-95 |
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| EDTA plasma (n=4) |
99 |
91-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2500 |
2.633 |
2.551 |
2.592 |
2.450 |
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| 1250 |
2.093 |
1.976 |
2.035 |
1.893 |
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| 625 |
1.398 |
1.345 |
1.372 |
1.230 |
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| 312 |
0.733 |
0.747 |
0.740 |
0.598 |
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| 156 |
0.456 |
0.494 |
0.475 |
0.333 |
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| 78 |
0.278 |
0.292 |
0.285 |
0.143 |
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| 39 |
0.221 |
0.217 |
0.219 |
0.077 |
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| 0 |
0.144 |
0.140 |
0.142 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
