Human NADPH oxidase 4(NOX4) ELISA kit
Product Details
| Target Name |
NADPH oxidase 4 |
| Alternative Names |
Kidney oxidase 1 ELISA Kit; Kidney oxidase-1 ELISA Kit; Kidney superoxide producing NADPH oxidase ELISA Kit; Kidney superoxide-producing NADPH oxidase ELISA Kit; KOX 1 ELISA Kit; KOX ELISA Kit; Kox-1 ELISA Kit; KOX1 ELISA Kit; NADPH ELISA Kit; NADPH oxidase 4 ELISA Kit; Nox4 ELISA Kit; NOX4_HUMAN ELISA Kit; Renal NAD(P)H oxidase ELISA Kit; Renal NAD(P)H-oxidase ELISA Kit; RENOX ELISA Kit |
| Abbreviation |
NOX4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
9.38 pg/mL-600 pg/mL |
| Sensitivity |
2.34 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NOX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
84 |
|
| Range % |
80-88 |
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| 1:10 |
Average % |
99 |
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| Range % |
95-103 |
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| 1:20 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:40 |
Average % |
107 |
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| Range % |
100-115 |
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| Recovery |
| The recovery of human NOX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
90 |
85-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 600 |
2.660 |
2.652 |
2.656 |
2.490 |
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| 300 |
2.194 |
2.075 |
2.135 |
1.969 |
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| 150 |
1.401 |
1.414 |
1.408 |
1.242 |
|
| 75 |
0.802 |
0.789 |
0.796 |
0.630 |
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| 37.5 |
0.469 |
0.486 |
0.478 |
0.312 |
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| 18.75 |
0.315 |
0.329 |
0.322 |
0.156 |
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| 9.38 |
0.208 |
0.216 |
0.212 |
0.046 |
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| 0 |
0.162 |
0.169 |
0.166 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
