Human Nerve growth factor,NGF ELISA kit

The Human Nerve growth factor (NGF) ELISA kit is designed for the detection and quantification of human NGF in serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. Many performances including specificity, sensitivity, precision, linearity, and recovery of this kit, have been rigorously tested. More validation information is elucidated in the product instructions. The NGF in the sample or standard is immobilized on the pre-coated NGF antibody and then sandwiched by Biotin-conjugated NGF antibody. The forming Ag-Ab-Ag immune complex is labeled by HRP-avidin and develops a color reaction upon adding TMB substrate solution. The color intensity is positively correlated to the amount of NGF bound in the initial step and can be measured at 450 nm via a microplate reader. NGF is a neurotrophic factor essential for the growth, differentiation, and survival of sympathetic and sensory afferent neurons during development. It contributes to neuronal phenotype by regulating ...Read more