Human Nesfatin-1 ELISA Kit
Product Details
| Target Name |
Nesfatin-1 |
| Abbreviation |
Nesfatin-1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
96 |
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| Range % |
80-105 |
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| 1:10 |
Average % |
99 |
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| Range % |
91-111 |
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| 1:20 |
Average % |
104 |
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| Range % |
92-112 |
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| 1:40 |
Average % |
93 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.617 |
2.557 |
2.587 |
2.463 |
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| 1000 |
2.205 |
2.356 |
2.281 |
2.157 |
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| 500 |
1.636 |
1.767 |
1.702 |
1.578 |
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| 250 |
1.043 |
1.058 |
1.051 |
0.927 |
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| 125 |
0.609 |
0.642 |
0.626 |
0.502 |
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| 62.5 |
0.352 |
0.346 |
0.349 |
0.225 |
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| 31.25 |
0.226 |
0.243 |
0.235 |
0.111 |
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| 0 |
0.122 |
0.125 |
0.124 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
