Human Neural -Cadherin, N-Cad ELISA kit
Product Details
| Target Name |
cadherin 2, type 1, N-cadherin (neuronal) |
| Alternative Names |
CADH2_HUMAN ELISA Kit; Cadherin 2 ELISA Kit; Cadherin 2 N cadherin neuronal ELISA Kit; Cadherin 2 type 1 ELISA Kit; Cadherin 2 type 1 N cadherin neuronal ELISA Kit; Cadherin 2; type 1; N-cadherin (neuronal) ELISA Kit; Cadherin-2 ELISA Kit; Cadherin2 ELISA Kit; Calcium dependent adhesion protein neuronal ELISA Kit; CD325 ELISA Kit; CD325 antigen ELISA Kit; CDH2 ELISA Kit; CDHN ELISA Kit; CDw325 ELISA Kit; CDw325 antigen ELISA Kit; N cadherin 1 ELISA Kit; N-cadherin ELISA Kit; NCAD ELISA Kit; Neural cadherin ELISA Kit; OTTHUMP00000066304 ELISA Kit; OTTHUMP00000067378 ELISA Kit |
| Abbreviation |
CDH2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 ng/mL-400 ng/mL |
| Sensitivity |
1.56 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human N-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
80-100 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-108 |
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| 1:8 |
Average % |
94 |
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| Range % |
85-98 |
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| Recovery |
| The recovery of human N-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-105 |
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| EDTA plasma (n=4) |
97 |
90-110 |
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| Typical Data |
 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.152 |
2.174 |
2.163 |
2.005 |
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| 200 |
1.832 |
1.824 |
1.828 |
1.670 |
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| 100 |
1.220 |
1.251 |
1.236 |
1.078 |
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| 50 |
0.926 |
0.974 |
0.950 |
0.792 |
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| 25 |
0.574 |
0.543 |
0.559 |
0.401 |
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| 12.5 |
0.406 |
0.426 |
0.416 |
0.258 |
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| 6.25 |
0.335 |
0.312 |
0.324 |
0.166 |
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| 0 |
0.152 |
0.164 |
0.158 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
