Human Neurofilament protein L (NF-L) ELISA kit
Product Details
| Description |
The product CSB-E16094h is a solid-phase ELISA kit for the detection and quantification of human Neurofilament protein L (NEFL) in serum, plasma, or cell culture supernates. NEFL, also referred to as NFL, is a subunit of neurofilaments (Nfs) and highly expressed in large-caliber myelinated axons. Its levels elevate in cerebrospinal fluid (CSF) and blood proportionally to the degree of axonal damage in various neurological disorders, including inflammatory, neurodegenerative, and cerebrovascular diseases. NFL is therefore a promising biomarker of neurodegeneration in the CSF and blood. The NEFL in the sample or standard is immobilized on the pre-coated NEFL antibody and then sandwiched by Biotin-conjugated NEFL antibody. The forming Ag-Ab-Ag immune complex is labeled by HRP-avidin and develops a color reaction upon adding TMB substrate solution. The color intensity is positively correlated to the amount of NEFL bound in the initial step and can be measured at 450 nm via a microplate rea... Read more |
| Target Name |
neurofilament, light polypeptide |
| Alternative Names |
68 kDa neurofilament protein ELISA Kit; 68kDa Neurofilament ELISA Kit; 68kDa neurofilament protein ELISA Kit; CMT1F ELISA Kit; CMT2E ELISA Kit; FLJ53642 ELISA Kit; Light molecular weight neurofilament protein ELISA Kit; NEFL ELISA Kit; Neurofilament light ELISA Kit; Neurofilament light polypeptide 68kDa ELISA Kit; Neurofilament light polypeptide ELISA Kit; Neurofilament protein, light chain ELISA Kit; Neurofilament subunit NF L ELISA Kit; Neurofilament triplet L protein ELISA Kit; NF-L ELISA Kit; NF68 ELISA Kit; NFL ELISA Kit; NFL_HUMAN ELISA Kit |
| Abbreviation |
NEFL |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NF-L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
99 |
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| Range % |
91-105 |
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| 1:10 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:20 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:40 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human NF-L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
98 |
92-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.770 |
2.665 |
2.718 |
2.628 |
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| 1000 |
2.114 |
2.006 |
2.060 |
1.970 |
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| 500 |
1.256 |
1.243 |
1.250 |
1.160 |
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| 250 |
0.657 |
0.651 |
0.654 |
0.564 |
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| 125 |
0.343 |
0.339 |
0.341 |
0.251 |
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| 62.5 |
0.252 |
0.242 |
0.247 |
0.157 |
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| 31.25 |
0.209 |
0.198 |
0.204 |
0.114 |
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| 0 |
0.092 |
0.088 |
0.090 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NEFL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled NEFL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
