Human Neurogranin(NRGN) ELISA kit
Product Details
| Description |
CUSABIO’s Neurogranin(NRGN) ELISA kit is specifically designed and validated for the quantitative detection of NRGN in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for neuroscience research use in humans. The kit has been validated with excellent sensitivity, high specificity, precision less than 10%, good linearity, high recovery, and tiny inter-batch differences. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which NRGN in the samples or standards are sandwiched between pre-coated NRGN antibody and Biotin-conjugated NRGN antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of NRGN bound in the initial step. The color development is stopped upon adding the stop solution, and the intensit... Read more |
| Alternative Names |
Protein kinase C substrate RC3 ELISA Kit; Calmodulin binding protein ELISA Kit; Hng ELISA Kit; NEUG(55-78) ELISA Kit; NEUG_HUMAN ELISA Kit; Neurogranin (protein kinase C substrate) ELISA Kit; Ng ELISA Kit; NRGN ELISA Kit; Protein kinase C substrate ELISA Kit; RC3 ELISA Kit |
| Abbreviation |
NRGN |
| Species |
Homo sapiens (Human) |
| Sample Types |
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| Detection Range |
|
| Sensitivity |
|
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NRGN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled NRGN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
