Product Details

Description

This Human Neuron-specific enolase (NSE) ELISA Kit allows for the in vitro quantitative determination of NSE concentrations in serum, plasma, and tissue homogenates. The assay uses the sandwich ELISA in which NSE in the sample is sandwiched between anti-human NSE antibody and Biotin-conjugated NSE antibody and the forming Ag-Ab-Ag complex is labeled by HRP-avidin and then develops color reaction after the addition of the TMB substrate solution. The color intensity is proportional to the amount of NSE in the sample and can be measured at 450 nm via a Microplate reader. The kit has undergone rigorous quality controlled in sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. NSE, also known as enolase 2 (ENO2), is a neuronal and neuroendocrine tissue-specific enolase isoenzyme. It is a critical glycolytic enzyme in the metabolic process of glycolysis. In the glycolytic penultimate step, ENO2 catalyzes the conversion of 2-phosphoglycerate (2-PGA) to phospho...Read more

Target Name

enolase 2 (gamma, neuronal)

Alternative Names

2 phospho D glycerate hydrolyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Eno 2 ELISA Kit; ENO2 ELISA Kit; ENOG ELISA Kit; ENOG_HUMAN ELISA Kit; Enolase 2 (gamma; neuronal) ELISA Kit; Enolase 2 ELISA Kit; Enolase 2 gamma neuronal ELISA Kit; Enolase2 ELISA Kit; Epididymis secretory protein Li 279 ELISA Kit; Gamma enolase ELISA Kit; Gamma-enolase ELISA Kit; HEL S 279 ELISA Kit; Neural enolase ELISA Kit; Neuron specific enolase ELISA Kit; Neuron specific gamma enolase ELISA Kit; Neuron-specific enolase ELISA Kit; neuronal enriched enolase ELISA Kit; Neurone specific enolase ELISA Kit; NSE ELISA Kit

Abbreviation

ENO2

Uniprot No.

P09104

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL 

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

• Intra-assay Precision (Precision within an assay): CV%
• Three samples of known concentration were tested twenty times on one plate to assess. 
• Inter-assay Precision (Precision between assays): CV%
• Three samples of known concentration were tested in twenty assays to assess.