Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit
Product Details
| Target Name |
nitric oxide synthase 1 (neuronal) |
| Alternative Names |
2310005C01Rik ELISA Kit; BNOS ELISA Kit; Constitutive NOS ELISA Kit; EC 1.14.13.39 ELISA Kit; IHPS 1 ELISA Kit; IHPS1 ELISA Kit; N-NOS ELISA Kit; NC-NOS ELISA Kit; neuronal Nitric Oxide Synthase ELISA Kit; Neuronal NOS ELISA Kit; Nitric oxide synthase ; neuronal; included ELISA Kit; Nitric oxide synthase 1 (neuronal) ELISA Kit; Nitric oxide synthase 1 ELISA Kit; Nitric oxide synthase; brain ELISA Kit; Nitric oxide synthase; penile neuronal; included ELISA Kit; NNOS ELISA Kit; NO ELISA Kit; NOS 1 ELISA Kit; NOS ELISA Kit; NOS type I ELISA Kit; NOS-I ELISA Kit; NOS1 ELISA Kit; NOS1_HUMAN ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit |
| Abbreviation |
NOS1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
0.156 IU/mL-10 IU/mL |
| Sensitivity |
0.039 IU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human nNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
87 |
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| Range % |
82-90 |
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| 1:10 |
Average % |
105 |
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| Range % |
101-110 |
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| 1:20 |
Average % |
83 |
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| Range % |
80-86 |
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| 1:40 |
Average % |
97 |
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| Range % |
91-103 |
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| Recovery |
| The recovery of human nNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| IU/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.902 |
2.963 |
2.933 |
2.743 |
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| 5 |
2.313 |
2.365 |
2.339 |
2.149 |
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| 2.5 |
1.469 |
1.465 |
1.467 |
1.277 |
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| 1.25 |
0.878 |
0.822 |
0.850 |
0.660 |
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| 0.625 |
0.568 |
0.545 |
0.557 |
0.367 |
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| 0.312 |
0.407 |
0.435 |
0.421 |
0.231 |
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| 0.156 |
0.314 |
0.326 |
0.320 |
0.130 |
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| 0 |
0.192 |
0.187 |
0.190 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
