Human neutrophil activating protein-2,NAP-2 ELISA Kit
Product Details
| Target Name |
Neutrophil-activating peptide 2 |
| Alternative Names |
B TG1 ELISA Kit; Beta TG ELISA Kit; Beta thromboglobulin ELISA Kit; Beta-TG ELISA Kit; C-X-C motif chemokine 7 ELISA Kit; Chemokine (C X C motif) ligand 7 ELISA Kit; Connective tissue activating peptide III ELISA Kit; CTAP 3 ELISA Kit; CTAP III ELISA Kit; CTAP-III ELISA Kit; CTAP-III(1-81) ELISA Kit; CTAP3 ELISA Kit; CTAPIII ELISA Kit; CXC chemokine ligand 7 ELISA Kit; CXCL 7 ELISA Kit; CXCL7 ELISA Kit; CXCL7_HUMAN ELISA Kit; LA PF 4 ELISA Kit; LA-PF4 ELISA Kit; LDGF ELISA Kit; Leukocyte derived growth factor ELISA Kit; Leukocyte-derived growth factor ELISA Kit; Low-affinity platelet factor IV ELISA Kit; Macrophage-derived growth factor ELISA Kit; MDGF ELISA Kit; NAP 2 ELISA Kit; NAP-2 ELISA Kit; NAP-2(1-63) ELISA Kit; NAP-2(1-66) ELISA Kit; NAP-2(73) ELISA Kit; NAP-2(74) ELISA Kit; Neutrophil activating peptide 2 ELISA Kit; Neutrophil-activating peptide 2(1-63) ELISA Kit; PBP ELISA Kit; Platelet basic protein ELISA Kit; PPBP ELISA Kit; Pro platelet basic protein (chemokine (C-X-C motif) ligand 7) ELISA Kit; Pro platelet basic protein ELISA Kit; SCYB7 ELISA Kit; Small inducible cytokine subfamily B member 7 ELISA Kit; Small-inducible cytokine B7 ELISA Kit; TC1 ELISA Kit; TC2 ELISA Kit; TGB ELISA Kit; TGB1 ELISA Kit; THBGB ELISA Kit; THBGB1 ELISA Kit; Thrombocidin 1 ELISA Kit; Thrombocidin 2 ELISA Kit; Thromboglobulin; beta-1 ELISA Kit |
| Abbreviation |
PPBP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
86 |
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| Range % |
83-89 |
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| 1:2000 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4000 |
Average % |
102 |
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| Range % |
97-106 |
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| 1:8000 |
Average % |
85 |
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| Range % |
81-89 |
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| Recovery |
| The recovery of human NAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-103 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.175 |
2.130 |
2.153 |
2.045 |
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| 2500 |
1.678 |
1.724 |
1.701 |
1.593 |
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| 1250 |
1.107 |
1.204 |
1.156 |
1.048 |
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| 625 |
0.762 |
0.754 |
0.758 |
0.650 |
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| 312 |
0.459 |
0.468 |
0.464 |
0.356 |
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| 156 |
0.305 |
0.315 |
0.310 |
0.202 |
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| 78 |
0.203 |
0.200 |
0.202 |
0.094 |
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| 0 |
0.110 |
0.105 |
0.108 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
