Human NGAL/MMP-9 complex ELISA kit
Product Details
| Target Name |
NGAL/MMP-9 complex |
| Abbreviation |
NGAL/MMP-9 complex |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, saliva, cell culture supernates, tissue homogenates |
| Detection Range |
0.313 ng/mL-20 ng/mL |
| Sensitivity |
0.029 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:10 |
Average % |
91 |
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| Range % |
87-98 |
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| 1:20 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:40 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:80 |
Average % |
94 |
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| Range % |
86-105 |
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| Recovery |
| The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
94-103 |
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| EDTA plasma (n=4) |
94 |
87-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
1.955 |
2.004 |
1.980 |
1.857 |
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| 10 |
1.637 |
1.629 |
1.633 |
1.510 |
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| 5 |
1.204 |
1.217 |
1.211 |
1.088 |
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| 2.5 |
0.894 |
0.907 |
0.901 |
0.778 |
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| 1.25 |
0.573 |
0.582 |
0.578 |
0.455 |
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| 0.625 |
0.416 |
0.424 |
0.420 |
0.297 |
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| 0.313 |
0.281 |
0.270 |
0.276 |
0.153 |
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| 0 |
0.124 |
0.121 |
0.123 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
