Human Nidogen 1 (NID1) ELISA Kit
Product Details
| Target Name |
nidogen 1 |
| Alternative Names |
Enactin ELISA Kit; Ent ELISA Kit; Entactin 1 ELISA Kit; Entactin ELISA Kit; NID 1 ELISA Kit; NID ELISA Kit; NID-1 ELISA Kit; Nid1 ELISA Kit; NID1_HUMAN ELISA Kit; Nidogen (enactin) ELISA Kit; Nidogen 1 ELISA Kit; Nidogen ELISA Kit; Nidogen-1 ELISA Kit |
| Abbreviation |
NID1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.39 ng/mL-25 ng/mL |
| Sensitivity |
0.09 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NID 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:8 |
Average % |
85 |
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| Range % |
82-93 |
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| Recovery |
| The recovery of human NID 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-95 |
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| EDTA plasma (n=4) |
101 |
96-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.186 |
2.140 |
2.163 |
2.016 |
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| 10 |
1.642 |
1.633 |
1.638 |
1.491 |
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| 5 |
1.251 |
1.220 |
1.236 |
1.089 |
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| 2.5 |
0.873 |
0.830 |
0.852 |
0.705 |
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| 1.25 |
0.579 |
0.541 |
0.560 |
0.413 |
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| 0.625 |
0.332 |
0.342 |
0.337 |
0.190 |
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| 0.312 |
0.260 |
0.265 |
0.263 |
0.116 |
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| 0 |
0.148 |
0.145 |
0.147 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
