Human Nuclear factor erythroid 2-related factor 2(NFE2L2) ELISA kit
Product Details
| Target Name |
nuclear factor (erythroid-derived 2)-like 2 |
| Alternative Names |
erythroid derived 2 ELISA Kit; HEBP1 ELISA Kit; like 2 ELISA Kit; NF E2 related factor 2 ELISA Kit; NF-E2-related factor 2 ELISA Kit; NF2L2_HUMAN ELISA Kit; NFE2 related factor 2 ELISA Kit; NFE2-related factor 2 ELISA Kit; Nfe2l2 ELISA Kit; Nrf 2 ELISA Kit; NRF2 ELISA Kit; Nuclear factor (erythroid derived 2) like 2 ELISA Kit; Nuclear factor ELISA Kit; nuclear factor erythroid 2 like 2 ELISA Kit; Nuclear factor erythroid 2 related factor 2 ELISA Kit; Nuclear factor erythroid 2-related factor 2 ELISA Kit; Nuclear factor erythroid derived 2 like 2 ELISA Kit |
| Abbreviation |
NFE2L2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates, cell lysates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NFE2L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
80-100 |
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| 1:2 |
Average % |
98 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
102 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-102 |
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| Recovery |
| The recovery of human NFE2L2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
82-104 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.336 |
2.352 |
2.344 |
2.187 |
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| 1000 |
1.787 |
1.776 |
1.782 |
1.625 |
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| 500 |
1.280 |
1.254 |
1.267 |
1.110 |
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| 250 |
0.845 |
0.821 |
0.833 |
0.676 |
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| 125 |
0.571 |
0.584 |
0.578 |
0.421 |
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| 62.5 |
0.364 |
0.359 |
0.362 |
0.205 |
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| 31.25 |
0.241 |
0.254 |
0.248 |
0.091 |
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| 0 |
0.156 |
0.158 |
0.157 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
