Human Nucleoside diphosphate kinase A(NME1) ELISA kit
Product Details
| Target Name |
non-metastatic cells 1, protein (NM23A) expressed in |
| Alternative Names |
AWD ELISA Kit; AWD, drosophila, homolog of ELISA Kit; GAAD ELISA Kit; Granzyme A activated DNase ELISA Kit; Granzyme A-activated DNase ELISA Kit; GZMA activated DNase ELISA Kit; Metastasis inhibition factor NM23 ELISA Kit; NB ELISA Kit; NBS ELISA Kit; NDK A ELISA Kit; NDKA ELISA Kit; NDKA_HUMAN ELISA Kit; NDP kinase A ELISA Kit; NDPK-A ELISA Kit; NDPKA ELISA Kit; NM23 ELISA Kit; NM23 long variant, included ELISA Kit; nm23-H1 ELISA Kit; NM23-M1 ELISA Kit; NM23H1B, included ELISA Kit; NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit; Nme1 ELISA Kit; NME1-NME2 spliced read-through transcript, included ELISA Kit; Non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit; Nonmetastatic cells 1, protein expressed in ELISA Kit; Nonmetastatic protein 23 ELISA Kit; Nonmetastatic protein 23, homolog 1 ELISA Kit; Nucleoside diphosphate kinase A ELISA Kit; Tumor metastatic process-associated protein ELISA Kit |
| Abbreviation |
NME1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
18.75 pg/mL-1200 pg/mL |
| Sensitivity |
4.68 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NME1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
96 |
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| Range % |
84-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
80-100 |
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| Recovery |
| The recovery of human NME1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
81-98 |
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| EDTA plasma (n=4) |
98 |
90-110 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1200 |
2.112 |
2.052 |
2.082 |
1.931 |
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| 600 |
1.849 |
1.706 |
1.778 |
1.627 |
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| 300 |
1.493 |
1.421 |
1.457 |
1.306 |
|
| 150 |
1.021 |
0.993 |
1.007 |
0.856 |
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| 75 |
0.578 |
0.554 |
0.566 |
0.415 |
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| 37.5 |
0.342 |
0.315 |
0.329 |
0.178 |
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| 18.75 |
0.231 |
0.219 |
0.225 |
0.074 |
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| 0 |
0.155 |
0.147 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
