| Target Name |
intelectin 1 (galactofuranose binding) |
| Alternative Names |
Endothelial lectin HL 1 ELISA Kit; Endothelial lectin HL-1 ELISA Kit; FLJ20022 ELISA Kit; Galactofuranose binding lectin ELISA Kit; Galactofuranose-binding lectin ELISA Kit; hIntL ELISA Kit; HL 1 ELISA Kit; HL-1 ELISA Kit; HL1 ELISA Kit; Intelectin ELISA Kit; Intelectin 1 ELISA Kit; Intelectin-1 ELISA Kit; Intestinal lactoferrin receptor ELISA Kit; INTL ELISA Kit; ITLN ELISA Kit; ITLN-1 ELISA Kit; ITLN1 ELISA Kit; ITLN1_HUMAN ELISA Kit; LFR ELISA Kit; Omentin ELISA Kit; OTTHUMP00000027882 ELISA Kit |
| Abbreviation |
ITLN1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human omentin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:200 |
Average % |
85 |
|
| Range % |
80-92 |
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| 1:400 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:800 |
Average % |
102 |
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| Range % |
93-110 |
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| 1:1600 |
Average % |
95 |
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| Range % |
82-99 |
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| Recovery |
| The recovery of human omentin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
80-100 |
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| EDTA plasma (n=4) |
96 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.349 |
2.192 |
2.271 |
2.166 |
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| 50 |
1.869 |
1.806 |
1.838 |
1.733 |
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| 25 |
1.354 |
1.294 |
1.324 |
1.219 |
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| 12.5 |
0.923 |
0.914 |
0.919 |
0.814 |
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| 6.25 |
0.592 |
0.559 |
0.576 |
0.471 |
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| 3.12 |
0.322 |
0.299 |
0.311 |
0.206 |
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| 1.56 |
0.213 |
0.209 |
0.211 |
0.106 |
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| 0 |
0.109 |
0.101 |
0.105 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
