| Target Name |
Orexin A |
| Alternative Names |
Hcrt ELISA Kit; Hcrt1 ELISA Kit; Hcrt2 ELISA Kit; Hypocretin ELISA Kit; Hypocretin neuropeptide precursor ELISA Kit; Hypocretin-1 ELISA Kit; Hypocretin-2 ELISA Kit; OREX_HUMAN ELISA Kit; Orexin ELISA Kit; Orexin precursor ELISA Kit; Orexin-B ELISA Kit; OX ELISA Kit; PPOX ELISA Kit |
| Abbreviation |
Orexin A |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
84 |
|
| Range % |
80-92 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:4 |
Average % |
84 |
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| Range % |
81-86 |
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| 1:8 |
Average % |
102 |
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| Range % |
100-106 |
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| Recovery |
| The recovery of human orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
87-96 |
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| EDTA plasma (n=4) |
97 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.334 |
2.556 |
2.445 |
2.341 |
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| 5 |
2.161 |
2.175 |
2.168 |
2.064 |
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| 2.5 |
1.645 |
1.756 |
1.701 |
1.597 |
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| 1.25 |
1.257 |
1.267 |
1.262 |
1.158 |
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| 0.625 |
0.818 |
0.886 |
0.852 |
0.748 |
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| 0.312 |
0.547 |
0.568 |
0.558 |
0.454 |
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| 0.156 |
0.331 |
0.356 |
0.344 |
0.240 |
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| 0 |
0.102 |
0.106 |
0.104 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
